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Applied Speciation offers ultra trace mercury speciation analyses of tissue and soil samples by extraction (EPA Method 3200) and analysis via ion chromatography cold vapor inductively coupled plasma mass spectrometry (IC-CV-ICP-MS). Extraction facilitating EPA Method 3200 allows for the quantitation of both methyl mercury (MMHg) and inorganic mercury [Hg(II)]. Chromatographic separation of the mercury species allows for the quantitation of both MMHg and Hg(II) in a single analytical run. Employing the cold vapor reaction prior to introduction into the ICP-MS yields an almost spectral free interference ion beam while reaching an almost 100% transfer efficiency of the mercury into the system. This equates to ultra trace detection limits (0.0005mg/kg) without the need for excessive sample dilution in most cases. This robust method of analysis allows for maximum automation which significantly decreases the per sample cost for analysis while increasing confidence in the results due to the capacity to perform mass balances.

Ultra low mercury speciation analysis of routine aqueous samples has been well established with EPA Method 1630 using derivatization followed by gas chromatography cold vapor atomic fluorescence spectrometry (GC-CV-AFS). Methyl mercury is not readily purged from the extract which requires derivatization (ethylation) with sodium tetraethyl borate (NaBEt₄). The reaction between the ethylating agent and methyl mercury is a non-specific reaction. The presence of other metals, organics, and other compounds can interfere with the derivatization efficiency producing a low bias result. The prevalence of this interference is low; however, quality control is rarely performed on every same which can result in biased results for more complicated matrices. 

The IC-CV-ICP-MS method employed at Applied Speciation eliminates the need for derivatization resulting in a more robust analytical method. Monitoring other mercury isotopes, inclusion of internal standards, and expanding the analyte list to include all dissolved mercury species equates to greater confidence in the data. The capability of quantitating MMHg and Hg (II) with IC-CV-ICP-MS also allows for monitoring species conversion during preparation and analysis which could not be accomplished with EPA Method 1630.

If you have a need for mercury speciation analysis, feel free to contact us to find out how Applied Speciation and Consulting can save you time and money with your next project (info@appliedspeciation.com).