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Applied Speciation offers a variety of analytical methods for arsenic speciation analysis. These methods utilize Ion Chromatography Coupled to an ICP-MS to achieve the lowest detection for different matrix types.

Arsenic speciation analysis was performed on different blood sources by ion chromatography inductively coupled plasma mass spectrometry to quantify arsenite, arsenate, monomethylarsonic acid, dimethylarsenic acid, arsenobetaine, arsenocholine, and other possible organic arsenic species.

Sample pretreatment was limited to dilution in ultra pure reagent water immediately followed by filtration (0.45mm) and analysis via IC-ICP-MS. Blood sources included Seronorm 201705 (a certified reference material) and a human test subject.

Quality control included a set of four (4) preparation blanks, a certified reference material (Seronorm 201705), matrix duplicate, one matrix spike and matrix spike duplicate set, one analytical duplicate, and one analytical spike and analytical spike duplicate. Total arsenic analysis via inductively coupled plasma dynamic reaction cell mass spectrometry was also performed for confirmation of the sum of species.

Arsenobetaine was the only quantifiable arsenic species present in the human blood donor while Seronorm 201705 contained both arsenobetaine and arsenite. The sum of species for the blood donor and Seronorm 201705 were 3.8 m g/kg and 27.1 m g/kg, respectively. The total arsenic results for both blood sources were very comparable to the sum of species which were 3.7 m g/kg and 25.9 m g/kg, respectively.

Although urine and epithelial tissues can provide better indication of long term arsenic exposure, the results form this investigation verifies that IC-ICP-MS is a viable option for arsenic speciation analysis in blood for acute arsenic exposure.

If you have any questions or would like a quotation, please feel free to email us at info@appliedspeciation.com or call (425) 483-3300.

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